Protein Variants | Comment | Organism |
---|---|---|
C270S | the replacement of any of the three conserved cysteine residues affords mutant proteins which are devoid of catalytic activity and contain less than 6% of Fe2+ and less than 23% of S2- as compared to the wild-type protein | Escherichia coli |
C273S | the replacement of any of the three conserved cysteine residues affords mutant proteins which are devoid of catalytic activity and contain less than 6% of Fe2+ and less than 23% of S2- as compared to the wild-type protein | Escherichia coli |
C306S | the replacement of any of the three conserved cysteine residues affords mutant proteins which are devoid of catalytic activity and contain less than 6% of Fe2+ and less than 23% of S2- as compared to the wild-type protein | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, 43% inhibition | Escherichia coli | |
Co2+ | 1 mM, 29% inhibition | Escherichia coli | |
EDTA | 10 mM, complete inhibition, reactivated by Mn2+ to a level of 25% | Escherichia coli | |
Fe2+ | 1 mM, 11% inhibition | Escherichia coli | |
Mg2+ | 1 mM, 25% inhibition | Escherichia coli | |
Ni2+ | 1 mM, 30% inhibition | Escherichia coli | |
Zn2+ | 1 mM, 98% inhibition | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
2-C-methyl-D-erythritol 2,4-cyclodiphosphate | pH 8.0, 37°C, photometric assay | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
iron-sulfur centre | the protein contained 2.4 iron ions and 4.4 sulfide ions per subunit | Escherichia coli | |
Mn2+ | reactivates EDTA-inactivated enzyme | Escherichia coli | |
additional information | the enzyme is active in absence of added metal ions | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
84000 | - |
x * 84000, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + reduced flavodoxin | Escherichia coli | the enzyme is involved in the nonmevalonate pathway of isoprenoid biosynthesis | (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + oxidized flavodoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P62620 | - |
- |
Purification (Comment) | Organism |
---|---|
the recombinant MalE/IspG fusion protein (expression level of about 40% with respect to the total soluble cell protein) is purified by affinity chromatography on an amylose resin FF column that is developed with a gradient of 0-10 mM maltose under anaerobic conditions, whereby the enzyme is enriched by a factor of 2.4 | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.045 | - |
activity with 2-C-methyl-D-erythritol 2,4-cyclodiphosphate and reduced deazaflavin, pH 8.0, 37°C | Escherichia coli |
0.099 | - |
activity with 2-C-methyl-D-erythritol 2,4-cyclodiphosphate and reduced flavodoxin, pH 8.0, 37°C, photometric assay | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + reduced deazaflavin | low activity with photoreduced deazaflavin as an artifactual electron donor | Escherichia coli | (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + oxidized deazaflavin | - |
? | |
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + reduced flavodoxin | the enzyme is involved in the nonmevalonate pathway of isoprenoid biosynthesis | Escherichia coli | (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + oxidized flavodoxin | - |
? | |
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + reduced flavodoxin | lower apparent activities are found using photoreduced deazaflavin as an artifactual electron donor, whereas dithionite is unable to serve as an artificial electron donor | Escherichia coli | (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + oxidized flavodoxin | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 84000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
IspG | ambiguous | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 9.5 | pH 8.0: about 65% of maximal activity, pH 9.5: about 75% of maximal activity | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
[4Fe-4S]-center | the protein contains 2.4 iron ions and 4.4 sulfide ions per subunit | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the nonmevalonate pathway of isoprenoid biosynthesis | Escherichia coli |